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Key Specifications Table
| Species Reactivity | Key Applications |
|---|---|
| H, M | WB, DB, ICC, ChIP |
| Description | |
|---|---|
| Catalogue Number | 17-656 |
| Trade Name |
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| Description | ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set |
| Alternate Names |
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| Background Information | All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Sox-2, clone 6F1.2 antibody/primer set includes the Sox-2, clone 6F1.2 antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 95 bp region within the promoter of the human nanog gene. The Sox-2, clone 6F1.2 antibody and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Sox-2-associated chromatin. Sox-2 plays a key role in embryonic stem cell development, and has been shown to help stem cells maintain pluripotent potential. It has also been shown to be associated with uncommitted dividing stem cell and precursor cells of the developing CNS. |
| Product Information | |
|---|---|
| Components |
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| Format | Purified |
| Control |
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| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This ChIPAb+ Sox-2, clone 6F1.2 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers. |
| Key Applications |
|
| Application Notes | Please refer to the EZ-Magna G ChIP™ (Cat. No. 17-409) or EZ-ChIP™ (Cat. No. 17-371) protocol for experimental details. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant GST Fusion protein. |
| Clone | 6F1.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes Sox-2, MW: ~34 kDa. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, mouse. Not tested in other species. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P48431 # Can form ternary complexes with OCT-1 or OCT-3 but acts as a transcriptional activator of FGF-4 enhancer DNA sequences only in conjunction with OCT-3 (By similarity). SIZE: 317 amino acids; 34310 Da SUBCELLULAR LOCATION: Nucleus. DISEASE: SwissProt: P48431 # Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. MCOPS3 is characterized by the association of microphthalmia or clinical anophthalmia with anomalies such as microcephaly, short stature, hypogonadotropic hypogonadism, esophageal atresia and neurologic manifestations. SIMILARITY: SwissProt: P48431 ## Contains 1 HMG box DNA-binding domain. |
| Molecular Weight | ~34 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Chromatin Immunoprecipitation: Sonicated chromatin prepared from Ntera 2 cells (3 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µg of either a normal mouse IgG, or Anti-Sox-2, clone 6F1.2 antibody and the Magna ChIP™ G Kit (Cat. No. 17-611). Successful immunoprecipitation of Sox-2-associated DNA fragments was verified by qPCR using ChIP Primers, nanog promoter (Figure 1). Please refer to the EZ-Magna ChIP™ G (Cat. No. 17-409) or EZ-ChIP™ (Cat. No. 17-371) protocol for experimental details. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 assays |
| Material Package | 25 assays per set. Recommended use: ~4 μg antibody per chromatin immunoprecipitation (dependent upon biological context). |