| Description | |
|---|---|
| Catalogue Number | 14-638 |
| Brand Family | Upstate |
| Trade Name |
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| Description | MLCK Protein, active, 10 µg |
| Overview | N-terminal 6His-tagged, recombinant, human MLCK, amino acids 1425-1771 |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal 6His-tagged, recombinant, human MLCK, amino acids 1425-1771, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed by baculovirus in Sf21 cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3 region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. |
| Gene Symbol |
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| Protein Target | MLCK |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | CAMK |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q15746 # Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. COFACTOR: Magnesium. & Calcium. SIZE: 1914 amino acids; 210774 Da SUBUNIT: All isoforms including Telokin bind calmodulin. TISSUE SPECIFICITY: Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues. PTM: MLCK is probably down-regulated by phosphorylation. SIMILARITY: SwissProt: Q15746 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 fibronectin type-III domain. & Contains 9 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain. |
| Molecular Weight | 43.7kDa |
| Product Usage Statements | |
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| Quality Assurance | routinely evaluated by phosphorylation of ZIPtide (Catalog # 12-545) |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 6 months at -70°C from date of shipment |
| Packaging Information | |
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| Material Size | 10 µg |