| Description | |
|---|---|
| Catalogue Number | 14-624 |
| Brand Family | Upstate |
| Trade Name |
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| Description | MST1 Protein, active, 10 µg |
| Overview | N-terminal His6-tagged, recombinant, full-length, human MST1 |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-terminal His6-tagged, recombinant, full-length, human MST1, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | baculovirus in Sf21 insect cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and its possible that this protein induces the chromatin condensation observed in this process. |
| Gene Symbol |
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| Protein Target | MST1 |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | STE |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q13043 # Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Phosphorylates Ser-14 of histone H2B during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. COFACTOR: Magnesium. SIZE: 487 amino acids; 55630 Da SUBUNIT: Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation. Interacts with and stabilizes SAV1. Interacts with RASSF1, which leads to enzyme activation. Interacts with FOXO3. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=The caspase-cleaved form cycles between the nucleus and cytoplasm. TISSUE SPECIFICITY: Ubiquitously expressed. PTM: Autophosphorylated on serine and threonine residues. SIMILARITY: SwissProt: Q13043 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 protein kinase domain. & Contains 1 SARAH domain. |
| Molecular Weight | 57kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of 250μM Axltide (KKSRGDYMTMQIG) |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
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| Material Size | 10 µg |