| Description | |
|---|---|
| Catalogue Number | 14-606 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | ASK1 Protein, active, 10 µg |
| Overview | N-Terminal GST and C-terminal His6-tagged, recombinant, human ASK1 amino acids 649-946 |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, N-Terminal GST & C-terminal His6-tagged, recombinant, human ASK1 amino acids 649-946, for use in Kinase Assays. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Source | expressed in E. coli cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
|
| Entrez Gene Summary | Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. |
| Gene Symbol |
|
| Protein Target | Ask1 |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | STE |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q99683 # Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death. COFACTOR: Magnesium. SIZE: 1374 amino acids; 154537 Da SUBUNIT: Homodimer when inactive. Binds both upstream activators and downstream substrates in multimolecular complexes. Associates with and inhibited by HIV-1 Nef. Interacts with DAB2IP. TISSUE SPECIFICITY: Abundantly expressed in heart and pancreas. SIMILARITY: SwissProt: Q99683 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 61.5kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of MBP |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -70°C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250 µg size --call for pricing and availability and reference catalog number 14-606M when ordering the 250 µg size. |