GenScript Dnase I; 1000U

Deoxyribonuclease I (DNase I) is DNA-specific endonuclease that cleaves both single-stranded DNA,double-stranded DNA and DNA-RNA hybrids, yielding 5-phosphate-terminated polynucleotides with a free hydroxyl group on position 3 producing tetranucleotides. The activity of DNase I is strictly dependent on Ca²⁺ and can be activated by divalent metal ions such as Mg²⁺ or Mn²⁺. In the presence of Mg²⁺, DNase I nonspecifically recognizes and cleaves a double-stranded DNA at anysite on either strand, and in the presence of Mn²⁺, it recognizes and cleaves almost the same sites on both strands of the DNA to produce DNA fragments with blunt ends or sticky ends with 1~2 nucleotide overhangs.

GenScript is offering DNase I produced by expression in a P. pastoris strain carrying a plasmid encoding the bovine DNase I.