| Description | |
|---|---|
| Catalogue Number | 12-493 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Abltide |
| Product Information | |
|---|---|
| Presentation | Lyophilized powder |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Key Applications |
|
| Biological Information | |
|---|---|
| Entrez Gene Number |
|
| Entrez Gene Summary | The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. |
| Gene Symbol |
|
| Protein Target | Abl |
| Target Sub-Family | TK |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P00519 # Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. COFACTOR: Magnesium or manganese. SIZE: 1130 amino acids; 122873 Da SUBUNIT: Interacts with SORBS1 following insulin stimulation. Found in a trimolecular complex containing CDK5 and CABLES1. Interacts with CABLES1 and PSTPIP1. Interacts with ZDHHC16 (By similarity). Interacts with INPPL1/SHIP2. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=The myristoylated c-ABL protein is reported to be nuclear. TISSUE SPECIFICITY: Widely expressed. PTM: Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation. Isoform IB is myristoylated on Gly-2. DISEASE: SwissProt: P00519 # A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). SIMILARITY: SwissProt: P00519 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated for use as a substrate for in vitro Abl-family kinase assays. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Lyophilized: 2 years at 4°C. Rehydrated: 6 months at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 1 mg |