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Immobilon® Signal Enhancer combines signal amplification and blocking in one ready-to-use reagent. Signal Enhancing: Amplify low signal intensity Western blots without amplifying the noise Save Primary Antibody: When the signal is strong, use Immobilon® Signal Enhancer to reduce the amount of valuable primary antibody required
Product Information
Detection method
Chemiluminescence
Quality Level
MQ300
Applications
Application
Western Blotting, immunodetection
Key Applications
Western Blotting
Application Notes
Immobilon® Signal Enhancer is designed for use in Western blotting applications when low signal intensity is observed. It combines signal amplification and blocking in one ready-to-use reagent. Enhancement of the antibody-antigen reaction can produce a 2 to 10-fold increase in signal intensity over traditionally used reagents. The reagent is formulated for use in both membrane blocking and antibody dilution steps and can be used for traditional immunodetection or with the SNAP i.d.® Protein Detection System. Immobilon® Signal Enhancer is compatible with PVDF as well as nitrocellulose blotting membranes and can be used with chromogenic, chemiluminescent, and fluorescent detection methods.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
