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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, R | IH(P), ICC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE184 |
| Description | Anti-Fox2 Antibody |
| Alternate Names |
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| Background Information | Fox2, also referred to as RNA binding protein fox-1 homolog 2, is an RNA binding protein involved in the regulation of tissue-specific alternative splicing. This protein interacts with the ER-alpha N-terminal activation domain, and contains a highly conserved RRM (RNA recognition motif), which associates with 5-UGCAUGU-3 elements. This protein has been demonstrated to regulate alternative splicing of tissue specific exons and differentially spliced exons during erythropoiesis. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-Fox2 Antibody (Rabbit Polyclonal Antibody) validated in IHC(P), ICC, WB to detect Fox2 also known as RNA binding protein fox-1 homolog 2, Fox-1 homolog B. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected Fox2 in human cerebellum tissue. Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected Fox2 in A431 cells. |
| Biological Information | |
|---|---|
| Immunogen | MBP-tagged recombinant protein corresponding to mouse Fox2. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human, Mouse, and Rat. Other homologies: Bovine (98% sequence homology), Canine (98% sequence homology), Equine (98% sequence homology). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: RNA-binding protein that regulates alternative splicing events by binding to 5-UGCAUGU-3 elements. Prevents binding of U2AF2 to the 3-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis. Seems to act as a coregulatory factor of ER-alpha. SUBUNIT STRUCTURE: Interacts with ER-alpha N-terminal activation domain. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. |
| Molecular Weight | ~48 kDa observed. Alternative splicing can result in up to 9 isoforms at various molecular weights. Uncharacterized bands between ~60-80 kDa have been observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in C2C12 cell lysate. Western Blot Analysis: 50 ng/mL of this antibody detected Fox2 in 10 µg of C2C12 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |