| Description | |
|---|---|
| Catalogue Number | 14-349 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | MAPKAP Kinase 2 Protein, inactive, 50 µg |
| Background Information | Recombinant human MAPKAP Kinase 2 (MAPKAP-K2), residues 46-400, A399G, containing an N-terminal GST tag MAPKAP Kinase 2 has been implicted as a cell cycle checkpoint kinase, joining the ranks of Chk1 and Chk2 as critical regulators of the DNA damage response in mammalian cells in recent studies (Manke, et al, 2005, and Abraham, 2005). This research indicates that MAPKAP Kinase 2, a serine/threonine kinase activated by p38, phosphorylates CDC25 B/C, generating a binding site for 14-3-3 proteins. The checkpoint kinases Chk1 and Chk2 are targets for drug discovery efforts designed to overcome cell cycle arrest due to DNA damage induced by chemotherapeutic agents. Overriding the checkpoint kinases in cancer cells exposed to DNA damaging agents leads to catastrophic failure of cell division and apoptosis. The new role of MAPKAP Kinase 2 in complementing the function of Chk1 and Chk2 suggests a new target for drug discovery efforts. |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Inactive, For use in Kinase Assays. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Source | Expressed in E.coli |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. |
| Gene Symbol |
|
| Protein Target | MAPKAP-K2 |
| Purification Method | Glutathione-agarose |
| Target Sub-Family | CAMK |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P49137 # Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at Ser-7 and tyrosine hydroxylase (on Ser-19 and Ser-40). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity. SIZE: 400 amino acids; 45568 Da SUBUNIT: Interacts with PHC2. TISSUE SPECIFICITY: Expressed in all tissues examined. PTM: Phosphorylated and activated by MAP kinase. SIMILARITY: SwissProt: P49137 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 protein kinase domain. |
| Molecular Weight | 66kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by phosphorylation of MAPKAP-K2 substrate peptide |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |