| Description | |
|---|---|
| Catalogue Number | 14-327 |
| Brand Family | Upstate |
| Trade Name |
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| Description | JNK1α1/SAPK1c Protein, active, 10 µg |
| Overview | Recombinant full-length human JNK1α1/SAPK1c with an N-terminal His-tag |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, recombinant full-length human JNK1α1/SAPK1c with an N-terminal His-tag, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Expressed in Sf21 cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. |
| Gene Symbol |
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| Protein Target | JNK1α1/SAPK1c |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | CMGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P45983 # JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta- 2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. COFACTOR: Magnesium. SIZE: 427 amino acids; 48296 Da SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP (By similarity). DOMAIN: SwissProt: P45983 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 45kDa |
| Product Usage Statements | |
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| Quality Assurance | Routinely evaluated by phosphorylation of ATF2 (amino acids 19-96) |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 1 year at -70°C |
| Packaging Information | |
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| Material Size | 10 µg |
| Material Package | 10 μg vial Also available in 250μg size (catalog number 14-327M). |