| Description | |
|---|---|
| Catalogue Number | 14-314 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Syk Protein, active, 10 µg |
| Overview | Recombinant full-length human Syk, containing an N-terminal His6-tag |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, recombinant full-length human Syk, containing an N-terminal His6-tag, for use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Expressed by baculovirus in Sf21 insect cells |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Gene Symbol |
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| Protein Target | Syk |
| Purification Method | Ni2+/NTA-agarose |
| Target Sub-Family | TK |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P43405 # Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion (By similarity). SIZE: 635 amino acids; 72066 Da SUBUNIT: Interacts with CBL and SLA when it is phosphorylated. The interaction with SLA may link it to CBL, leading to its destruction. Interacts with phosphorylated NFAM1 (By similarity). Interacts with Epstein-Barr virus LMP2A. Interacts through its SH2 domains with the phosphorylated ITAM domain of CD79A which stimulates SYK autophosphorylation and activation. PTM: Autophosphorylated. & Phosphorylation on Tyr-323 creates a binding site for c-Cbl, an adapter protein that serves as a negative regulator of BCR- stimulated calcium ion signaling (By similarity). & Phosphorylation on Tyr-348 and Tyr-352 enhances the phosphorylation and activation of phospholipase C-gamma and the early phase of calcium ion mobilization via a phosphoinositide 3- kinase-independent pathway (By similarity). & Ubiquitinated by CBLB after BCR activation; which promotes proteasomal degradation (By similarity). SIMILARITY: SwissProt: P43405 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains. |
| Molecular Weight | 73kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by phosphorylation of Poly (Glu4-Tyr) |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 1 year at -70C |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | Also available in 250μg size -- call for pricing and availability and reference catalog number 14-314M when ordering the 250μg size. |