| Description | |
|---|---|
| Catalogue Number | 14-197 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Casein Kinase 2 Protein, active, 10 µg |
| Overview | Recombinant full-length human Casein Kinase 2 protein, containing an &alpyha;-subunit N-terminal 6His tag and a β-subunit N-terminal GST tag. |
| Product Information | |
|---|---|
| Presentation | 20mM MOPS, pH 7.4, 1mM EDTA, 0.1% (v/v) Triton X-100, 0.3% 2-mercaptoethanol, 1mM benzamidine, 50% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Active, recombinant full-length human Casein Kinase 2 protein, containing an α-subunit N-terminal 6His tag & a β-subunit N-terminal GST tag. For use in Kinase Assays. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Expressed in Sf21 insect cells. |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. |
| Gene Symbol |
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| Protein Target | CK2 |
| Purification Method | Sequential chromatography using heparin Sepharose™ and Mono Q anion exchange resin |
| Target Sub-Family | CMGC |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P68400 # Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates Ser-392 of p53/TP53 following UV irradiation. SIZE: 391 amino acids; 45144 Da SUBUNIT: Tetramer composed of an alpha chain, an alpha and two beta chains. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Interacts with RNPS1. SIMILARITY: SwissProt: P68400 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | heterotetramer of 49kDa α-subunit and 53kDa β-subunit (α2β2) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by phosphorylation of the Casein Kinase 2 Substrate Peptide. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 1 year at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |
| Material Package | 2 vials at 5μg each Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-197M when ordering the 250μg size. |