| Description | |
|---|---|
| Catalogue Number | 12-338 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Wortmannin, (PI3 Kinase inhibitor) |
| Overview | Wortmannin, a fungal metabolite, is a cell-permeable, irreversible inhibitor of phosphatidylinositol 3 kinase (PI3 Kinase) with an IC50 of 5nM. It blocks the catalytic activity of PI3-Kinase without affecting upstream signaling events such as insulin receptor tyrosine kinase activity. Wortmannin also inhibits the activities of myosin light chain kinase and PI4-Kinase at concentrations a hundred times higher than those required to inhibit PI3-Kinase. |
| Product Information | |
|---|---|
| Presentation | 1mg of lyophilized powder packaged under inert gas. White to off-white powder. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | The Wortmannin, (PI3 Kinase inhibitor) controls the biological activity of PI3 Kinase. This small molecule/inhibitor is primarily used for Biochemicals applications. |
| Key Applications |
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| Biological Information | |
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| Entrez Gene Number |
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| Entrez Gene Summary | Receptor-regulated class I phosphoinositide 3-kinases (PI3Ks) phosphorylate the membrane lipid phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to PtdIns(3,4,5)P3, which in turn recruits and activates cytosolic effectors involved in proliferation, survival, or chemotaxis. PIK3R5 is a PI3K regulatory subunit (Brock et al., 2003 [PubMed 12507995]).[supplied by OMIM] |
| Gene Symbol |
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| Protein Target | PI3Kγ |
| Target Sub-Family | PI3K/PI4K |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q8WYR1 # Regulatory subunit of the PI3K gamma complex. SIZE: 880 amino acids; 97348 Da SUBUNIT: Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit. Interacts with G beta gamma proteins. SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity). TISSUE SPECIFICITY: Highly expressed in leukocytes, followed by spleen, lymph node, thymus ans bone marrow. DOMAIN:SwissProt: Q8WYR1 The heterodimerization region allows the binding to the catalytic subunit. SIMILARITY: |
| Product Usage Statements | |
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| Quality Assurance | Routinely evaluated by kinase assay. Purity: ≥95% by HPLC. Molecular Formula: C23H24O8 Molecular Weight: 428.4 |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Protect from light. Stable for 2 years as supplied at -20°C. Following reconstitution aliquot and store at -20°. The DMSO/ethanol stock solutions are stable for 1-3 months at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 1 mg |