GenScript Cho-K1/Pac1/Gα15 Stable Cell Line; 2vials

List Price $8,925.00 Your Price $8,925.00
GenScript Cho-K1/Pac1/Gα15 Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART: GSCRPT-M00203
MFG.PART: M00203
UNSPSC: 41105322
Manufacturer: GenScript USA Inc
Description

The Vasoactive intestinal Peptide/Pituitary Adenylate Cyclase Activating Polypeptide (VPAC) receptor is G protein-coupled and has been divided into at least three types: PAC1, VPAC1, and VPAC2. High expression of PAC1 is observed in CNS and the adrenal medulla. Several splice variants of PAC1 result in proteins that differ at the N-terminus and third intracellular loop; these variants differ in their affinities for PACAP and abilities to activate Gq and Gs. This manual describes establishment of a cell line and a protocol of pharmacologically validated human PAC1 GPCR receptor (Genebank Accession Number: NM_003382).

Synonyms

Human Recombinant Vasoactive Intestinal Polypeptide Receptor Stable Cell Line

Overview
Applications Functional assay for PAC1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B (Cat. # 10687010, Invitrogen), 400 μg/ml G418 (Cat. #10131-035, Gibco)


More Information
SKU GSCRPT-M00203
Featured No
Supplier Part Number M00203
UM EA
UNSPSC 41105322
Manufacturer Name GenScript USA Inc
Temperature Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
ProductLine GSCRPT
Qty 1
MinOrderQty 1
Weight 7.000000
Lead Time 7
Hazardous N
Energy Star No
Green No
ACT Ecolabel No
Controlled N
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