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GenScript Cgmp Elisa Detection Kit; 1plate (8 wells x 12 strips)
List Price
$297.00
Your Price
$297.00
GenScript Cgmp Elisa Detection Kit - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-L00461
MFG.PART:
L00461
UNSPSC:
41105322
Manufacturer:
GenScript USA Inc
Description
Kit DescriptionGenScript cGMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cGMP (Guanosine 3,5-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. cGMP is an important secondary messenger in signal transduction pathways. It is a common regulator of ion channel conductance, glycogenolysis, and cellular apoptosis. cGMP is a cyclic nucleotide derived from guanosine triphosphate (GTP). The cellular production of cGMP is mediated by Guanylate cyclase (GC). It activates the cGMP-dependent protein kinases which in turn phosphorylate several downstream protein targets. cGMP has an effect on the regulation of cAMP levels by activating or inhibiting specific phosphodiesterases (PDEs). The anti-IgG Capture Plate is pre-coated with fixed amount of Goat anti-rabbit IgG to capture Rabbit Anti-cGMP Polyclonal Antibody. When free cGMP or specimen and HRP-cGMP conjugate are added to the well, they compete in the solution to interact with the cGMP antibody captured on the plate. Other unbound molecules are removed by a wash step. The cGMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the amount of cGMP present in the unknown samples can be calculated by transforming its absorbance value. |
Overview
| Key Features | Key FeaturesApplicationQuantitative detection of cGMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine. StorageThe unopened kit is stable for at least 12 months if stored at 2-8 °C, and the opened kit is stable for up to 2 weeksat 2-8 °C. Do not freeze the kit. Kit Components
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Product Introduction
| Storage | Store at 4°C |
| SKU | GSCRPT-L00461 |
|---|---|
| Featured | No |
| Supplier Part Number | L00461 |
| UM | EA |
| UNSPSC | 41105322 |
| Manufacturer Name | GenScript USA Inc |
| Temperature | Store at 4°C |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | Y |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |