The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
GenScript Anti-His Affinity Resin is designed for purification of His-tagged protein. It is composed of agarose and conjugated THETM anti-His monoclonal antibody. His-tagged protein in the crude samples can bind to the anti-His monoclonal antibodies coupled with the agarose, and the nonspecific proteins can be eliminated by washing. As a result, the His-tagged protein will finally be eluted with high recovery. Moreover, this product can be used for immunoprecipitation of His-tagged protein.
Note
Note:
The product is designed for purification and immunoprecipitation of His-tagged protein, especially for the sample failed by Ni-NTA Agarose Chromatography purification.
Overview
Key Features
Characteristics:
Features
Specifications
Beads structure
4% agarose
Beads size
45-165 μm, averagely 90 μm
Coupled antibody
THETM His Tag Antibody, mAb, Mouse (Cat.No A00186)
Binding capacity
More than 0.6 mg of His-tagged protein per mL settled resin
Specificity
N-terminal/C-terminal/internal/4 x His/5 x His/6 x His-tagged protein
Stability
Stable at 2-8 °C for 1 year
Storage
2-8 °C. Avoid freezing the resin
Repeat usage
Generally the resin can be recycled at least 4 times. If maintained properly, the resin may be recycled 10 times with minor binding capability loss
Elution methods
Acid, alkaline and peptide competitive elutions
Reagents compatibility
Compatible with mostly used reagents at certain concentrations
Consistency
Lot-to-lot consistency proved by strict production procedure