GenScript Ni-Charged Magbeads; 8mL (2 mL Settled Beads)

Name: GenScript Ni-Charged Magbeads; 8mL (2 mL Settled Beads)
SKU: GSCRPT-L00295
Price: 101.00 USD

List Price $101.00 Your Price $101.00

GenScript Ni-Charged Magbeads - GSCRPT (Additional S&H or Hazmat Fees May Apply)

NETA PART: GSCRPT-L00295

MFG.PART: L00295

UNSPSC: 41105322

Manufacturer: GenScript USA Inc

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Details

Description

The GenScript Ni-Charged MagBeads are developed for quick and efficient small-scale purification of polyhistidine-tagged proteins under native or denaturing conditions.

Perform cell lysis under native or denaturing conditions. Add the cell lysate containing your polyhistidine-tagged proteins to the Ni-Charged MagBeads and allow the proteins bind to the MagBeads. Then the isolated proteins can be eluted from the beads. Magnetic separation eliminates the need for multiple tubes, minimizes the loss of sample and removes several steps of the centrifugation process.

The GenScript Ni-Charged MagBeads are average 40 µm in size, super paramagnetic beads with strong metal-chelating agent covalently bound to their surfaces. They are pre-charged with nickel and ready to use for quick and small-scale purification of polyhistidine-tagged proteins. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol and 1 mM NiSO₄. The Ni-Charged MagBeads have a binding capacity of 40 mg 6×His-tagged protein per 1 mL settled beads (e.g. 4 mL 25% slurry).

Overview

Key Features

Key Features

Quick and convenient separation accomplished by magnetic force
High binding capacity with 40 mg of 6xHis-tagged protein/ml settled beads
Compatible with various reagents needed in the purification process (see table 1)
Can be regenerated for multiple uses
Extremely low nonspecific binding

Table 1 Reagents Compatible with Ni-charged Magnetic Beads

Denaturants	Detergents	Reducing agents	Salts	Others
6 M Gu•HCl	2% Triton X-100	20 mM β-ME	4 M MgCl₂	50% glycerol
8 M Urea	2% Tween 20	1 mM DTT	5 mM CaCl₂	20% ethanol
	1% CHAPS		2 M NaCl	1 mM EDTA

Properties

Storage

This product is stable until the expiration date stated on the COA, when stored unopened at 2–8°C. Do NOT freeze the product. Keep the MagBeads in liquid suspension during storage and all handling steps. Drying will cause loss of binding capacity and result in reduced performance. Resuspend the beads well before use. Be careful to avoid bacterial/fungal contamination.

Specifications

More Information
SKU	GSCRPT-L00295
Featured	No
Supplier Part Number	L00295
UM	EA
UNSPSC	41105322
Manufacturer Name	GenScript USA Inc
Temperature	This product is stable until the expiration date stated on the COA, when stored unopened at 2– 8°C . Do NOT freeze the product . Keep the MagBeads in liquid suspension during storage and all handling steps. Drying will cause loss of binding capacity and r
ProductLine	GSCRPT
Qty	1
MinOrderQty	1
Weight	7.000000
Lead Time	7
Hazardous	N
Energy Star	No
Green	No
ACT Ecolabel	No
Controlled	N

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GenScript Ni-Charged Magbeads; 8mL (2 mL Settled Beads)

Key Features

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