Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1128 |
| Description | Anti-SETBP1 Antibody, clone 63 |
| Alternate Names |
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| Background Information | SET-binding protein (UniProt Q9Y6X0; also known as SEB, SETBP1) is encoded by the SETBP1 (also known as KIAA0437, MRD29) gene (Gene ID 26040) in human. SETBP1 is shown to bind the SET nuclear oncogene involved in DNA replication. It is expressed in various tissues with low levels in myeloid and monocytic cells and in CD34+ cells, however, higher expression is observed in myeloid malignancies. Overexpression of SETBP1 promotes leukemogenesis by enhancing full-length SET protein and then impairing the phosphatase activity of the tumor suppressor PP2A through the formation of a SETBP1-SET-PP2A complex. Patients with SETBP1 overexpression have a significantly shorter overall survival, and poor prognosis, particularly in the elderly patients. Mutations in SETBP1 gene are associated with Schinzel-Giedion midface retraction syndrome, which is characterized by severe mental retardation, distinctive facial features, and multiple congenital malformations. (Ref.: Cristobal, I., et al. (2010). Blood 115(3): 615-625). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SETBP1, clone 63, Cat. No. MABE1128, is a highly specific mouse monoclonal antibody that targets SETBP1 and has been tested in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:4 dilution of clone 63 hybridoma culture supernatant detected SETBP1 in 30-50 µg of REH human acute lymphoid leukemia cell lysate (Courtesy of Jesper Christensen, Ph.D. and Kristian Helin, Ph.D., University of Copenhagen, Denmark). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human SETBP1 N-terminal fragment. |
| Clone | 63 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 63 targets the N-terminal region of human and mouse SETBP1. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~175 kDa observed. 175.0 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected SETBP1 in 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |