Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1074 |
| Description | Anti-SMARCA3 (HLTF) Antibody, clone 11-1 |
| Alternate Names |
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| Background Information | Helicase-like transcription factor (UniProt Q14527; also known as DNA-binding protein/plasminogen activator inhibitor 1 regulator, HIP116, RING finger protein 80, Sucrose non-fermenting protein 2-like 3, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3) is encoded by the HLTF (also known as HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1) gene (Gene ID 6596) in human. Helicase-like transcription factor (115 kDa) is a SWI/SNF (mating-type switching/sucrose non-fermenting) protein that uses ATP hydrolysis to remodel chromatin in multiple cellular processes. It is shown to regulate transcription by binding to the SV40 enhancer and the promoters Serpin E1 and HIV-1. It is well expressed in brain, heart, kidney, liver, lung, pancreas, placenta, and skeletal muscle. It was initially characterized for its DNA binding and transcriptional activity, however, later was shown to act as an E3 ubiquitin ligase and was implicated in post-replication DNA repair by polyubiquitination of proliferating cell nuclear antigen (PCNA). The inactivation of HIP116 is shown to induce an elevated level of chromosome breaks and fusions in mouse embryonic fibroblasts after treatment with alkylating agents. HIP116 inactivation in transgenic mice is reported to disrupt cell cycle at the G2/M phase. Ref.: Arcolia, V et al. (2014). BMC Cancer 14, 492. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SMARCA3 (HLTF), clone 11-1, Cat. No. MABE1074, is a highly specific mouse monoclonal antibody that targets HTLF and has been tested in Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected predominantly nuclear HLTF (SMARCA3) immunoreactivity in human cervical cancer and cerebellum tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human HLTF (SMARCA3) N-terminal fragment. |
| Clone | 11-1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 11-1 detected predominantly nuclear HLTF immunoreactivity by immunohistochemistry staining. Clone 11-1 epitope lies within the N-terminal region present in human HLTF (SMARCA3) spliced isoform 1, but not in isoform 2 reported by UniProt (Q14527). |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | 113.9 kDa (human isoform 1) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human breast cancer tissue section. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected predominantly nuclear HLTF (SMARCA3) immunoreactivity in human breast cancer tissue section. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |