Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | ICC, WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1614 |
| Description | Anti-AATF Antibody, clone 1B2D8 |
| Alternate Names |
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| Background Information | Protein AATF (UniProt: Q9JKX4; also known as Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1, Traube protein) is encoded by the Aatf (also known as Che1, Trb) gene (Gene ID: 56321) in murine species. AATF is a nuclear protein that functions as a general inhibitor of the histone deacetylase HDAC1. It binds to the pocket region of RB1 to displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. AATF is expressed in adrenal gland, Purkinje cells, heart, kidney, liver, lung, muscle, ovary, and testis. It is expressed uniformly throughout the embryo until E10.5. However, from E11.5, the relative expression level increases in the liver, hind brain, spinal cord, dorsal root ganglia, and the posterior commissure. Down-regulation of AATF is involved in colon carcinoma cell proliferation and over-expression of Aatf gene interferes with MAP3K12 induced apoptosis. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-AATF, clone 1B2D8, Cat. No. MABC1614, is a rat monoclonal antibody that detects AATF and it has been tested for use in Western Blotting and Immunocytochemistry. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot detected AATF in Immunocytochemistry applications (Ishigaki, S., et. al. (2010). Cell Death Differ. 17(5):774-86). Western Blotting Analysis: A representative lot detected AATF in Western Blotting applications (Ishigaki, S., et. al. (2010). Cell Death Differ. 17(5):774-86). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the first 39 amino acids from murine Apoptosis-antagonizing transcription factor (AATF). |
| Clone | 1B2D8 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 1B2D8 is a rat monoclonal antibody that detects AATF in murine and human cells. It targets an epitope within the first 39 amino acids from the N-terminal region. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~79 kDa observed; 59.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected AATF in 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |