Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, IHC, FUNC, WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1613 |
| Description | Anti-MUC1 Antibody, clone HMFG2 |
| Alternate Names |
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| Background Information | Mucin-1 (UniProt: P15941; MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227) is encoded by the MUC1 (also known as PUM) gene (gene ID: 4582) in human. The MUC-1 alpha subunit has cell adhesive properties and functions as protective layer on epithelial cells against bacteria and enzymes. It can act as both cell adhesion and an anti-adhesion protein. It forms a tight, non-covalent heterodimeric complex with the proteolytically-released beta-subunit. The beta subunit contains a C-terminal domain that is involved in cell signaling via phosphorylations and protein-protein interactions. It modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, it impacts directly or indirectly the Ras/MAPK pathway. MUC-1 also is involved in tumor progression and transcription through regulation of Tp53. MUC-1 is expressed in epithelial cells, breast, uterus, activated and unactivated T-cells. It is shown to be over-expressed in epithelial tumors, such as breast or ovarian cancer. MUC-1 is exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, it is internalized and recycled to the cell membrane. MUC-1 is highly glycosylated (N- and O-linked carbohydrates and sialic acid) and is O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Its interaction, via the tandem repeat region, with domain 1 of ICAM1 is implicated in cell migration and metastases. MUC1 serves as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence. Lower levels over time may be indicative of a positive response to treatment. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Mucin-1 using this mouse monoclonal Anti-MUC1, clone HMFG2, Cat. No. MABC1613 that has been tested for use in Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Functional studies, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MUC1 in human lung and human pancreas tissue. Western Blotting Analysis: A representative lot detected MUC1 in Western Blotting applications (Kaur, S., et. al. (2014). PLoS One. 9(3):e92742; Schroeder, J.A., et. al. (2001). J Biol Chem. 276(16):13057-64). Affects Function: A representative lot detected MUC1 in Affects Function applications (Schettini, J., et. al. (2012). Cancer Immunol Immunother. 61(11):2055-65). Immunoprecipitation Analysis: A representative lot detected MUC1 in Immunoprecipitation applications in (Schroeder, J.A., et. al. (2001). J Biol Chem. 276(16):13057-64). Immunohistochemistry Analysis: A representative lot detected MUC1 in Immunohistochemistry applications in (Sakurai, J., et. al. (2007). Eur J Histochem. 51(2):95-102). |
| Biological Information | |
|---|---|
| Immunogen | Delipidated milk fat globule extract followed 3 weeks later by epithelial cells cultured from milk (for 2 weeks). |
| Clone | HMFG2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone HMFG2 immunostains human breast cancer tissue. It can react with the unglycosylated peptide as well as several glycoforms of MUC1. |
| Isotype | IgG1λ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 122.10 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human breast cancer tissue. Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected MUC1 in human breast cancer tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |