Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IHC, WB, IP, IF | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1592 |
| Description | Anti-SAP-1 Antibody, clone 123 |
| Alternate Names |
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| Background Information | Receptor type protein tyrosine phosphatase sap-1 (UniProt: B6ZDS3; also known as Stomach-cancer associated protein tyrosine phosphatase 1, SAP-1) is encoded by Ptprh (also known as Sap1) gene (Gene ID: 545902) in murine species. SAP-1 is a receptor-type (R3) protein tyrosine phosphatase that is expressed predominantly in the gastrointestinal tract, most prominently in the microvilli of the brush border in gastrointestinal epithelial cells. SAP-1 has a single catalytic domain in its cytoplasmic region and eight fibronectin type III-like domains in its extracellular region. It is synthesized with a 27 amino acid signal peptide, which is subsequently cleaved. SAP-1 is reported to play a significant role in the regulation of intestinal tumorigenesis and its expression is markedly increased in colon and pancreatic cancers. Ablation of SAP-1 in IL-10-deficient mice display greater severity of colitis in association with up-regulation of mRNAs for various cytokines and chemokines in the colon. The cytoplasmic region of SAP-1 is shown to bind directly to Lck. Over-expression of SAP-1 results in down-regulation of Lck activity that leads to inhibition of T cell receptor-mediated T cell function. (Ref.: Murata, Y et al. (2015). Proc. Natl. Acad. Sci. USA. 112(31):E4264-71). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect SAP1 using this rat monoclonal Anti-SAP-1 Antibody, clone 123, Cat. No. MABC1592. It has been tested in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected SAP-1 in HEK293A cells transfected with murine SAP-1 versus mock control. (Courtesy of Yoji Murata, Ph.D. and Takashi Matozaki, M.D., Ph.D. Kobe University Graduate School of Medicine, Japan). Immunofluorescence Analysis: A representative lot detected SAP-1 in Immunofluorescence applications (Sadakata, H., et. al. (2009). Genes Cells. 14(3):295-308; Murata, Y., et. al. (2015). Proc Natl Acad Sci USA. 112(31):E4264-71). Immunofluorescence Analysis: A representative lot detected SAP-1 in ileum from 8-week old WT mouse (Courtesy of Yoji Murata, Ph.D. and Takashi Matozaki, M.D., Ph.D. Kobe University Graduate School of Medicine, Japan). Immunoprecipitation Analysis: A representative lot detected SAP-1 in Immunoprecipitation applications (Murata, Y., et. al. (2015). Proc Natl Acad Sci USA. 112(31):E4264-71). Immunohistochemistry Analysis: A representative lot detected SAP-1 in Immunohistochemistry applications (Sadakata, H., et. al. (2009). Genes Cells. 14(3):295-308; Murata, Y., et. al. (2015). Proc Natl Acad Sci USA. 112(31):E4264-71). Western Blotting Analysis: A representative lot detected SAP-1 in Western Blotting applications (Sadakata, H., et. al. (2009). Genes Cells. 14(3):295-308; Murata, Y., et. al. (2015). Proc Natl Acad Sci USA. 112(31):E4264-71). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant fusion protein comprising the extracellular region of mouse SAP-1 (amino acids 1-604) and the Fc region of human IgG. |
| Epitope | extracellular domain |
| Clone | 123 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 123 recognizes SAP-1 in HEK293A cells transfected with murine SAP-1. It targets an epitope in the extracellular domain. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Mouse. Not Human. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~180 kDa observed; 106.98 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293A cells transfected with murine SAP-1. Western Blotting Analysis: 1 µg/mL of this antibody detected SAP-1 in 10 µg of lysate from HEK293A cells transfected with murine SAP-1. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |