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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC, IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1205 |
| Description | Anti-UDP-Galactose Transporter Antibody, clone 1H10.1 |
| Alternate Names |
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| Background Information | UDP-galactose translocator (UniProt P78381; also known as Solute carrier family 35 member A2, UDP-Gal-Tr, UDP-galactose transporter, UDP-galactose transporter, UGT) is encoded by the SLC35A2 (also known as CDG2M, UGALT, UGT, UGTL) gene (Gene ID 7355) in human. UGT is a multi-pass membrane protein that transports UDP-galactose from the cytosol into the Golgi vesicles where it serves as a glycosyl donor for the generation of glycans. Its short N-terminal region, composed of 35 amino-acid residues, is reported to be crucial for galactosylation of N-glycans. Mutations in SLC35A2 gene are known to reduce UDP-galactose transport that leads to galactose-deficient glycoproteins resulting in developmental delays, hyotonia, ocular anomalies, and brain malfunctions. Higher expression of UGT mRNA has been reported in human colon cancer. Ref.: Ng, BG., et al. (2013). Am. J. Human Gen. 92, 632-636. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect UDP-galactose transporter (SLC35A2) using this mouse monoclonal Anti-UDP-Galactose Transporter, clone 1H10.1, Cat. No. MABC1205, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: Clone 1H10.1 hybridoma culture supernatant immunostained Golgi apparatus in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized A431 cells. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected UDP-Gal transporter in human colon, liver, lung, and placenta tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human UDP-Gal transporter C-terminal end sequence. |
| Clone | 1H10.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | The immunogen sequence is 100% present in spliced isoforms 1 (UGT2) and 3, while being only partially present in spliced isoforms 2 (UGT1), 4, and 5 reported by UniProt (P78381), reactivity toward these latter isoforms has not been confirmed. |
| Isotype | IgG3κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~50 kDa observed. 14.31/25.18 k.Da (isoform 1/3) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human placenta tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UDP-Gal Transporter in 10 µg of human placenta tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |