Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1187 |
| Description | Anti-Apaf-1 Antibody, clone 18H2 |
| Alternate Names |
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| Background Information | Apoptotic protease-activating factor 1 (UniProt: O88879; also known as Apaf-1) is encoded by the Apaf-1 gene (Gene ID: 11783) in murine species. Apaf-1 contains a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. It is highly expressed in lung and spleen, weakly in brain and kidney and is not detectable in liver. Under basal conditions it is a monomeric protein, but oligomerizes under apoptotic conditions. The monomeric form is auto-inhibited in a closed conformation through a bound ADP at the nucleotide binding site. Under apoptosis signals ADP is exchanged for ATP and its binding to cytochrome c triggers a conformation change where WD repeat region swings out, which allows the NB-ARC domain to rotate and expose the contact areas for oligomerization. Oligomeric form is a heptameric ring that binds to cytochrome c and ATP and is known as the apoptosome. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 to mature caspase-9, which then activates effector caspases 3, 6, and 7. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2a in PBS with 0.02% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Apaf-1 Antibody, clone 18H2, Cat. No. MABC1187, is a rat monoclonal antibody that specifically detects Apaf-1. It has been tested for use in Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Scott, C.L., et. al. (2004). J Cell Biol. 164(1):89-96). Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Marsden, V.S., et. al. (2006). Blood. 107(5):1872-7). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant fragment corresponding to first 97 amino acids from the N-terminal region of murine Apaf-1. |
| Epitope | N-terminus |
| Clone | 18H2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 18H2 detects Apaf-1 in human and mouse cells. It targets an epitope within the first 97 amino acids from the N-terminal region containing the CARD domain. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~130 kDa observed;141 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in SH-SY5Y cell lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Apaf-1 in 10 mg of SH-SY5Y cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |