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Millipore Complex Of N-Terminal 6his-Tagged, Recombinant, Full-Length Human P110α Containing The Mutation E545k & Untagged, Recombinant, Full-Length Human P85α. For Use In Kinase Assays. - Mill (Additional S&H or Hazmat Fees May Apply)
Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E545K and untagged, recombinant, full-length human p85α.
Background Information
The E545K substitution is a somatic mutation in p110α that has been associated with tumours of the colon and brain. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation.
Product Information
Quality Level
MQ100
Applications
Application
Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E545K & untagged, recombinant, full-length human p85α. For use in Kinase Assays.
Key Applications
Kinase Assay
Biological Information
Source
Coexpressed by baculovirus in Sf21 insect cells.
Specific Activity
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
NM_006218.2
Entrez Gene Summary
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Gene Symbol
PIK3CA
PI3K
MGC142161
p110-alpha
MGC142163
Protein Target
PI3Kα/p85α
Purification Method
Ni2+/NTA-agarose
Target Sub-Family
PI3K/PI4K
UniProt Number
P42336
UniProt Summary
FUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. SIZE: 1068 amino acids; 124284 Da SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity). DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370]. SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
Molecular Weight
p110α (E545K) MW = 125.3kDa, p85α MW = 83.7kDa.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Packaging Information
Material Size
10 µg
Material Package
Also available in 250 μg size --call for pricing and availability and reference catalog number 14-783M when ordering the 250 μg size.
