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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IP, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL582 |
| Replaces | CBL1322 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-CD19 Antibody, clone HD37 |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CD19 Antibody, clone HD37 detects level of CD19 & has been published & validated for use in FC, IP, IH. |
| Key Applications |
|
| Application Notes | Identification of CD19 positive B lymphocytes and malignant B cells by indirect immunofluorescence staining Leukaemia and lymphoma phenotyping Identification of normal and malignant CD19 positive B lymphocytes in cryostat frozen sections Immunoprecipitation of the CD19 antigen Studies of the CD19/21 complex in B cell activation and the control of B cell development Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Clone | HD37 |
| Host | Mouse |
| Specificity | The CD19 antigen (90 kDa) is expressed from the earliest stages of B-progenitor development, on all peripheral B cells including germinal centre B cells and all B cell lines and B cell leukaemias tested. T cell and monocytic cell lines are negative and the antigen is lost on B cell maturations to plasma cells. The antigen is a type I integral membrane glycoprotein whose in vitro inhibition will influence B cell activation and proliferation. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P15391 # Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. SIZE: 556 amino acids; 61128 Da SUBUNIT: Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE:SwissProt: P15391 # Defects in CD19 are a cause of hypogammaglobulinemia [MIM:107265]. SIMILARITY: SwissProt: P15391 ## Contains 2 Ig-like C2-type (immunoglobulin-like) domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |