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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Am, B, H, Po, R | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL188 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Clathrin Antibody, clone CHC5.9 |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | The antibody was purified by gel filtration and is presented as 100μg of lyophilysed material. Reconstitute with 1ml distilled water. Final buffer composition is PBS, pH 7.4. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Clathrin Antibody, clone CHC5.9 is an antibody against Clathrin for use in WB, IH. |
| Key Applications |
|
| Application Notes | Marker for detection of receptor mediated endocytosis Suitable for frozen sections Western blotting KNOW SPECIES Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Coated vesicles (clathrin) of bovine brain. |
| Clone | CHC5.9 |
| Host | Mouse |
| Specificity | This antibody reacts with the clathrin heavy chain from a diverse range of species. In nervous tissue it cross-reacts with another coated vesicle polypeptide of 190 kDa which is not present in isolated clathrin triskelions. In some epithelial cells of certain species the antibody may cross-react with a cytokeratin component. It detects coated vesicles from different organs and tissues (e.g. brain, ovary, mammary gland). : Recognizes bovine, rat and amphibian |
| Isotype | IgM |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q00610 # Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. SIZE: 1675 amino acids; 191615 Da SUBUNIT: Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium. SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Cytoplasmic side. Membrane, coated pit; Cytoplasmic side. Melanosome. Note=Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. SIMILARITY: SwissProt: Q00610 ## Belongs to the clathrin heavy chain family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C for up to 12 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |