Key Specifications Table
| Key Applications |
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| Cell Based Assays |
| Description | |
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| Catalogue Number | SCT047 |
| Description | BioTracker Cystine-FITC Live Cell Dye |
| Overview | Uptake of cystine allows accumulation of cysteine that is necessary for glutathione synthesis and reactive oxygen species (ROS) detoxification. After the uptake of cystine into the cytoplasm, the reducing intracellular environment leads to a separation of one cystine molecule into two cysteine molecules. A key function of cysteine is to maintain the cellular pools of glutathione (GSH). Cystine uptake is also required for T-cell activation and B-cell development. The BioTracker Cystine-FITC dye is a live cell green fluorescent imaging probe that measures cystine uptake at the single cell level. The probe has been used to measure T-cell activation using traditional and imaging flow cytometry methods. Spectral Properties Absorbance: 450nm Emission: 525nm References Rathmell JC et al. Fluorescence-based measurement of cystine uptake through xCT shows requirement for ROS detoxification in activated lymphocytes. J Immunol Methods. 2016 Nov;438:51-58. |
| Alternate Names |
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| Product Information | |
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| Detection method | Fluorescence |
| Presentation | Lyophilized |
| Applications | |
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| Application | Live cell imaging dye that measures cystine uptake at the single cell level used to detect glutathione (GSH) synthesis, reactive oxygen species (ROS) detoxification, T-cell activation and B-cell development. |
| Key Applications |
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| Application Notes | Live cell fluorescent imaging |
| Product Usage Statements | |
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| Quality Assurance | Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis Molar Mass: 625.65 g/mol |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Store BioTracker Cystine-FITC Live Cell Dye at -20°C, desiccate and protect from light Note: Centrifuge vial briefly to collect contents at bottom of vial before opening. |
| Packaging Information | |
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| Material Size | 1 mg |