Key Specifications Table
| Key Applications |
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| Cell Based Assays |
| Description | |
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| Catalogue Number | SCT045 |
| Description | BioTracker ATP-Red Live Cell Dye |
| Overview | Adenosine triphosphate (ATP) is the primary energy source for all cellular processes. ATP also functions as a signaling molecule for regulating cell movement, neurotransmission and ion channel functions. ATP is localized in mitochondria, where cellular respiration occurs. ATP levels can be used to measure cell proliferation and cell cycle dynamics. The BioTracker ATP-Red dye is a live cell red fluorescent imaging probe for adenosine triphosphate (ATP). The probe targets ATP specifically in the mitochondria of living cells. The probe shows no cross reactivity to numerous analytes including: Zn2+, Mg2+, Ca2+, Na2+, K+, GSH, HOCL, H2O2, arabinose, galactose, glucose, fructose, ribose, sorbose, sucrose, xylose, heparin, AMP, ADP, CMP, CDP, CTP, UMP, UDP, UTP, GMP, GDP or GTP. The probe is non-fluorescent when forming a closed ring structure. In the presence of the negatively charged ATP, the covalent bonds between boron and ribose is broken and the ring opens producing fluorescence. Spectral Properties Absorbance: 510nm Emission: 570nm References Chang YT et al. A Multisite‐Binding Switchable Fluorescent Probe for Monitoring Mitochondrial ATP Level Fluctuation in Live Cells. Angew Chem Int Ed Engl. 2016 Jan 26;55(5):1773-6. |
| Alternate Names |
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| Product Information | |
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| Detection method | Fluorescence |
| Presentation | Lyophilized |
| Applications | |
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| Application | Live cell imaging dye for cellular adenosine triphosphate (ATP) localized to mitochondria used to detect metabolic activity, cell proliferation and overall cell health. |
| Key Applications |
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| Application Notes | Live cell fluorescent imaging |
| Product Usage Statements | |
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| Quality Assurance | Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis Molar Mass: 561.48 g/mol |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Store BioTracker ATP-Red Live Cell Dye at -20°C, desiccate and protect from light Note: Centrifuge vial briefly to collect contents at bottom of vial before opening. |
| Packaging Information | |
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| Material Size | 1 mg |