Cell Signaling Lamp1 (E5n9z) Rabbit mAb

Name: Cell Signaling Lamp1 (E5n9z) Rabbit mAb
SKU: CSIG-99437S
Price: 342.39 USD

List Price $342.39 Your Price $342.39

Cell Signaling Lamp1 (E5n9z) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-99437S

MFG.PART: 99437S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	M
Sensitivity	Endogenous
Molecular Weight (kDa)	42 (non-glycosylated), 90-120 (glycosylated)
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunofluorescence (Frozen): 1:50 - 1:100, Immunofluorescence (Immunocytochemistry): 1:100 - 1:400}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	LAMP1 (E5N9Z) Rabbit mAb recognizes endogenous levels of total LAMP1 protein.
Species Reactivity	Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse LAMP1 protein.
Background	Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6).

Specifications