Cell Signaling Cleaved Caspase-3 (Asp175) (5a1e) Rabbit mAb (Bsa And Azide Free)

Name: Cell Signaling Cleaved Caspase-3 (Asp175) (5a1e) Rabbit mAb (Bsa And Azide Free)
SKU: CSIG-94530SF
Price: 884.76 USD

List Price $884.76 Your Price $884.76

Cell Signaling Cleaved Caspase-3 (Asp175) (5a1e) Rabbit mAb (Bsa And Azide Free) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-94530SF

MFG.PART: 94530SF

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µg
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	17, 19
Source/Isotype	Rabbit IgG
Application/Dilution	{}
Storage	Supplied in 1X PBS, BSA and Azide Free.For standard formulation of this product see product #9664.
Specificity/Sensitivity	Cleaved Caspase-3 (Asp175) (5A1) Rabbit mAb (BSA and Azide Free) detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases. Non-specific labeling may be observed by immunofluorescence in specific sub-types of healthy cells in fixed-frozen tissues (e.g. pancreatic alpha-cells). Cytoplasmic background may be observed in human and monkey samples.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3.
Background	Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).

Specifications