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Cell Signaling Phospho-Bad (Ser112) (40a9) Rabbit mAb (Bsa And Azide Free)
List Price
$884.76
Your Price
$884.76
Cell Signaling Phospho-Bad (Ser112) (40a9) Rabbit mAb (Bsa And Azide Free) - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-94223SF
MFG.PART:
94223SF
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µg |
| Reactivity | H M R Mk |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 23 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {} |
| Storage | Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #5284. |
| Specificity/Sensitivity | Phospho-Bad (Ser112) (40A9) Rabbit mAb (BSA and Azide Free) detects endogenous levels of Bad only when phosphorylated at Ser112. The Ser112 nomenclature is based upon the mouse sequence. The analogous phosphorylation site is Ser75 in human and Ser113 in rat. This antibody does not detect Bad phosphorylated at other sites, nor does it detect related family members. |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser112 of mouse Bad protein. |
| Background | Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). |
| SKU | CSIG-94223SF |
|---|---|
| Featured | No |
| Supplier Part Number | 94223SF |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |