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Cell Signaling Pi31/Psmf1 Antibody
List Price
$309.06
Your Price
$309.06
Cell Signaling Pi31/Psmf1 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-91788S
MFG.PART:
91788S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H M R |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 28, 29 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | PI31/PSMF1 Antibody recognizes endogenous levels of total PI31/PSMF1 protein. |
| Species Reactivity | Human, Mouse, Rat |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro245 of human PI31/PSMF1 protein. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | Proteasome-binding protein PI31 (Proteasomal Inhibitor of 31kD) is encoded by the PSMF1 gene and regulates proteasome function in the cell, which is essential for cell function and survival. Regulated degradation of intracellular proteins is mediated by the ubiquitin-proteasome system (UPS) by a multi-step process (1,2). First, protein to be degraded is tagged with a chain of ubiquitin molecules. Ubiquitinated proteins are then cleaved into small peptides by the 26S proteasome. Proteasome activity by the UPS is regulated at additional levels, including transcriptional expression of proteasomal proteins, posttranslational modification, and by various regulatory proteins. PI31/PSMF1 is a structurally conserved proteasome regulator that binds to the 20S proteasome and functions to inhibit 20S proteasome hydrolysis of small peptides (3,4). Unexpectedly, PI31/PSMF1 promotes UPS-mediated protein breakdown in vivo, suggesting additional proteasome regulatory functions beyond inhibition of the 20S proteasome (5). PI31/PSMF1 also serves as an adaptor protein to transport proteasomes via cellular protein motors (6,7). This process may be particularly important in neurons where local proteasomal degradation at distal tips of neurons may play a critical role in the maintenance of neuronal architecture, circuitry, and function. |
| SKU | CSIG-91788S |
|---|---|
| Featured | No |
| Supplier Part Number | 91788S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |