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Cell Signaling Nono Antibody
List Price
$309.06
Your Price
$309.06
Cell Signaling Nono Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-90336S
MFG.PART:
90336S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H M R Mk |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 60 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:50 - 1:200} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | NONO Antibody recognizes endogenous levels of total NONO protein. |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NONO protein. Antibodies are purified by peptide affinity chromatography. |
| Background | Non-POU domain-containing octamer-binding protein (NONO), also referred to as p54nrb, is a DNA and RNA-binding protein that is involved in several nuclear processes. NONO binds to the conventional octamer duplex of double-stranded DNA, and can additionally bind to single-stranded DNA and RNA at a location independent of the duplex site (1). NONO belongs to the Drosophila melanogaster behavior, human splicing (DBHS) protein family along with paraspeckle protein 1 (PSPC1) and splicing factor, proline- and glutamine-rich (PSF/SFPQ) (2). NONO, PSPC1, and PSF/SFPQ associate together along with long nonprotein-coding RNA NEAT1 to form ribonucleic protein bodies referred to as paraspeckles, which play a role in gene expression and regulation (2). NONO heterodimerizes with PSF/SFPQ to process pri-miRNA prior to presentation to the Drosha complex (3). The NONO/PSF heterodimer can also stabilize paired DNA ends and promote DNA double-strand break repair by canonical non-homologous end rejoining (4). NONO also forms a complex with PSF/SFPQ and DExH-box helicase 9 (DHX9) that produces a long isoform of BIN1, promoting carcinogenesis in hepatocellular carcinoma (5). In breast cancer, NONO regulates pre-mRNA splicing and binding to mRNAs of cell proliferation-related genes, regardless of hormone sensitivity (6). NONO has also been hypothesized to play a role in heart disease, and a recent study in mice showed that NONO deficiency led to heart defects by fibroblast hyperproliferation (7). |
| SKU | CSIG-90336S |
|---|---|
| Featured | No |
| Supplier Part Number | 90336S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |