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Cell Signaling Phospho-Stat1 (Tyr701) (58d6) Rabbit mAb (Pe-Cy7 ® Conjugate)
List Price
$481.77
Your Price
$481.77
Cell Signaling Phospho-Stat1 (Tyr701) (58d6) Rabbit mAb (Pe-Cy7 ® Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-90145S
MFG.PART:
90145S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl (50 tests) |
| Reactivity | H M |
| Sensitivity | Endogenous |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Flow Cytometry (Fixed/Permeabilized): 1:50} |
| Storage | Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. |
| Specificity/Sensitivity | Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (PE-Cy7® Conjugate) detects endogenous levels of Stat1 only when phosphorylated at Tyr701. The antibody detects phosphorylated Tyr701 of p91 Stat1 and also the p84 splice variant. It does not cross-react with the corresponding phospho-tyrosines of other Stat proteins. |
| Species Reactivity | Human, Mouse |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr701 of human Stat1. |
| Background | The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. |
| SKU | CSIG-90145S |
|---|---|
| Featured | No |
| Supplier Part Number | 90145S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | +4C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |