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Cell Signaling Trib1 (E6m3t) Rabbit mAb
List Price
$374.71
Your Price
$374.71
Cell Signaling Trib1 (E6m3t) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-87592S
MFG.PART:
87592S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 50 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:200} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | TRIB1 (E6M3T) Rabbit mAb recognizes endogenous levels of total TRIB1 protein. This antibody is not predicted to cross-react with other TRIBBLES proteins, based on sequence divergence in the epitope region. |
| Species Reactivity | Human |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TRIB1 protein. |
| Background | TRIBBLES proteins belong to a small family of serine-threonine kinase-like proteins characterized by the presence of a variant protein kinase motif (lacking a canonical ATP binding site), a MEK1 binding site, and a C-terminal COP1 site that binds ubiquitin ligase. The tribbles gene was first identified and characterized in Drosophila genetic screens for genes that regulate cell division, gastrulation, and oogenesis (1-3). Research studies in Drosophila suggested that TRIBBLES functions to coordinate cell division by regulating turnover of the cell cycle protein String/cdc25. In contrast to the Drosophila genome, which contains a single tribbles gene, the genomes of mice and humans encode three known TRIBBLES proteins (TRIB1-3), which exhibit both distinct and overlapping patterns of expression and functions (4). For example, TRIB1 and TRIB2, but not TRIB3, were reported to promote degradation of the basic region-leucine zipper transcription factor C/EBPα, a function that appears to be conserved from flies to humans (5,6). TRIB2 is overexpressed in a subset of human AML patient samples, downregulated in leukemic cells undergoing proliferation arrest (7), and positively regulated by the NOTCH signaling pathway in T cells (8), while retroviral-mediated overexpression of TRIB2 in mice was shown to induce transplantable leukemia (7). These findings collectively suggest that TRIB2 functions as an oncogene in the mammalian hematopoietic system (9). |
| SKU | CSIG-87592S |
|---|---|
| Featured | No |
| Supplier Part Number | 87592S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |