We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Cell Signaling 3x Dykddddk Tag (E7c5t) Rabbit mAb
List Price
$342.39
Your Price
$342.39
Cell Signaling 3x Dykddddk Tag (E7c5t) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-87537S
MFG.PART:
87537S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | All |
| Sensitivity | Transfected Only |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | 3X DYKDDDDK Tag (E7C5T) Rabbit mAb detects exogenously expressed proteins containing the 3X DYKDDDDK epitope tag. This antibody recognizes the 3X DYKDDDDK tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells, but does not detect DYKDDDDK tag. 3X DYKDDDDK Tag (E7C5T) Rabbit mAb detects exogenously expressed 3X DYKDDDDK-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. |
| Species Reactivity | All Species Expected |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the 3X DYKDDDDK epitope (DYKDHDGDYKDHDIDYKDDDDK). |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1). |
| SKU | CSIG-87537S |
|---|---|
| Featured | No |
| Supplier Part Number | 87537S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |