Cell Signaling Lamin A (133a2) Mouse mAb

Name: Cell Signaling Lamin A (133a2) Mouse mAb
SKU: CSIG-86846S
Price: 339.36 USD

List Price $339.36 Your Price $339.36

Cell Signaling Lamin A (133a2) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-86846S

MFG.PART: 86846S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µg
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	74
Source/Isotype	Mouse IgG3
Application/Dilution	{Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:1000 - 1:4000, Immunofluorescence (Immunocytochemistry): 1:2000}
Storage	Supplied at 1 mg/mL in PBS containing 0.09% sodium azide. Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance. This product is stable for 36 months when stored at -20C.
Specificity/Sensitivity	Lamin A (133A2) Mouse mAb recognizes endogenous levels of total lamin A protein. It also reacts with large fragments of lamin A (50 kDa) produced by caspase cleavage during apoptosis. This antibody does not cross-react with lamin C.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human lamin A protein.
Background	Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear dysregulation and cell death (5,6).

Specifications