Cell Signaling P14 Arf (E3x6d) Rabbit mAb (Alexa Fluor 647 Conjugate)

Name: Cell Signaling P14 Arf (E3x6d) Rabbit mAb (Alexa Fluor 647 Conjugate)
SKU: CSIG-86802S
Price: 454.50 USD

List Price $454.50 Your Price $454.50

Cell Signaling P14 Arf (E3x6d) Rabbit mAb (Alexa Fluor 647 Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-86802S

MFG.PART: 86802S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl (50 tests)
Reactivity	H
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Immunofluorescence (Immunocytochemistry): 1:50, Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity/Sensitivity	p14 ARF (E3X6D) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total p14 ARF protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro126 of human p14 ARF protein.
Background	Human p14 ARF (p19 ARF in mouse) is a pro-apoptotic cell cycle regulator frequently inactive in human tumors (1). Basal expression of p14 ARF is low in most cell types, but accumulation of this protein occurs in response to oncogene expression (2,3). Increased p14 ARF levels facilitate MDM2 degradation, leading to increased p53 protein levels and subsequent cell cycle arrest and/or apoptosis (4). While most p14 ARF signaling has traditionally thought to be p53-dependent, more recent reports have described p53-independent p14 ARF signaling leading to cell cycle arrest and apoptosis (5,6).

Specifications