Cell Signaling Paf15 (D8e2y) Xp Rabbit mAb

Name: Cell Signaling Paf15 (D8e2y) Xp Rabbit mAb
SKU: CSIG-81533T
Price: 200.99 USD

List Price $200.99 Your Price $200.99

Cell Signaling Paf15 (D8e2y) Xp Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-81533T

MFG.PART: 81533T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	15
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:150}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	PAF15 (D8E2Y) XP® Rabbit mAb recognizes endogenous levels of total PAF15 protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PAF15 protein.
Background	Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). PCNA associated factor (PAF15) interacts with PCNA to recruit DNA replicative polymerase. In response to DNA damage, PCNA recruits a low fidelity DNA polymerase to allow bypass of lesions that would otherwise stall DNA replication. This form of DNA damage tolerance is called translesion synthesis (TLS), and is regulated in part by PAF15 (2,3). Ubiquitination of PAF15 at lysines 15 and 24 regulates its interactions with PCNA and DNA polymerase (3). PAF15 is overexpressed in human cancers, and research studies show a correlation between PAF15 expression and poor prognosis (3,4).

Specifications