Cell Signaling Vsv-G Tag Antibody

Name: Cell Signaling Vsv-G Tag Antibody
SKU: CSIG-81454S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Vsv-G Tag Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-81454S

MFG.PART: 81454S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	All
Sensitivity	Transfected Only
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:400}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	VSV-G Tag Antibody detects recombinant proteins containing the VSV-G epitope tag. The antibody recognizes the VSV-G tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody detects a non-specific band of unknown origin at 65 kDa.
Species Reactivity	All Species Expected
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide containing the vesicular stomatitis virus glycoprotein epitope (YTDIEMNRLGK). Antibodies are purified by peptide affinity chromatography.
Background	Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The VSV-G tag is derived from an epitope from the C-terminal region of vesicular stomatitis virus glycoprotein (1). VSV-G is widely used both as an epitope tag in expression vectors and for pseudotyping lentiviral vectors due to the stability and broad host range of the resulting pseudotypes (2,3).

Specifications