Cell Signaling U2af2 (E6m3g) Rabbit mAb

Name: Cell Signaling U2af2 (E6m3g) Rabbit mAb
SKU: CSIG-79462S
Price: 342.39 USD

List Price $342.39 Your Price $342.39

Cell Signaling U2af2 (E6m3g) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-79462S

MFG.PART: 79462S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	43, 46, 65
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	U2AF2 (E6M3G) Rabbit mAb recognizes endogenous levels of total U2AF2 protein. This antibody recognizes U2AF2 protein cleavage products around 43 and 46 kDa.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val275 of human U2AF2 protein.
Background	U2 small nuclear RNA auxiliary factor 2 (U2AF2) is the large (65 kDa) subunit of the U2 auxiliary factor (U2AF) that plays an essential role in the splicing of pre-mRNA to generate functional mRNA transcripts. U2AF2 forms a heterodimer with the small (35 kDa) U2AF1 subunit to create the U2AF that recognizes the 3 splice site and facilitates spliceosome assembly (1-3). Research studies indicate that U2AF1 binds to the 3 splice site consensus AG dinucleotide at the intron-exon boundary while U2AF2 recognizes and binds the polypyrimidine tract upstream of the 3’ splice site. These two steps ensure accurate spliceosome assembly at splice sites (4-6). Mutations in U2AF2, which alter its splicing capabilities, have been described in various cancers (7,8).

Specifications