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Cell Signaling Spastin Antibody
List Price
$309.06
Your Price
$309.06
Cell Signaling Spastin Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-79310S
MFG.PART:
79310S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H M R |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 45-60 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Spastin Antibody recognizes endogenous levels of total Spastin protein. |
| Species Reactivity | Human, Mouse, Rat |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln226 of human Spastin protein. Antibodies are purified by peptide affinity chromatography. |
| Background | Spastin (SPG4 or SPST) is a member of the ATPases associated with multiple cellular activities (AAA) protein family that contains a microtubule interacting and organelle transport (MIT) domain. There are four spastin isoforms, which are imported into the nucleus. One of the isoforms contains two nuclear export signals that efficiently drive export to the cytoplasm (1,2). Spastin is involved in severing microtubules that are polyglutaminated and also regulates microtubule abundance and mobility (3). Spastin promotes endosomal tubule fission by the interaction of the ER-localized spastin isoform M1-spastin and the endosomal IST1 protein at ER-endosome contacts. Rescue of endosomal tubulation phenotypes requires spastins microtubule-severing ATPase capacity and its ability to bind the endosomal proteins IST1 and CHMP1B (4). Spastin regulates microtubule stability to modulate synaptic structure and function, and it is essential for the synaptic formation and axonal growth in developing neurons (1,5). Spastin is linked to several diseases including Hereditary Spastic Paraplegia (HSP) that affects upper motor neurons within the tubular ER membrane in corticospinal neurons (6). Some data suggest that spastin interacts with Lewy bodies and liquid droplets in Parkinsons disease (7). The missorting of Tau in Alzheimers disease is strongly related to the formation of Tau oligomers and aggregates, and induces a mislocalization of TTLL6 (Tubulin-Tyrosine-Ligase-Like 6). TTLL6 induces the polyglutamylation of microtubules that triggers spastin-mediated severing of dendritic microtubules (8). |
| SKU | CSIG-79310S |
|---|---|
| Featured | No |
| Supplier Part Number | 79310S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |