Cell Signaling Apex2 (E5a2z) Rabbit mAb

Name: Cell Signaling Apex2 (E5a2z) Rabbit mAb
SKU: CSIG-74728S
Price: 342.39 USD

List Price $342.39 Your Price $342.39

Cell Signaling Apex2 (E5a2z) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-74728S

MFG.PART: 74728S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M
Sensitivity	Endogenous
Molecular Weight (kDa)	62
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	APEX2 (E5A2Z) Rabbit mAb recognizes endogenous levels of total APEX2 protein. This antibody cross-reacts with an aproximately 100 kDa band of unknown origin by western blot.
Species Reactivity	Human, Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu393 of human APEX2 protein.
Background	DNA-(apurinic or apyrimidinic site) endonuclease 2 (APEX2/APE2) is an endodeoxyribonuclease that functions in the DNA base excision repair (BER) pathway to repair DNA lesions induced by oxidative and alkylating agents. APEX2 acts in ATR-dependent DNA damage checkpoint activation in response to oxidative stress (1). The APEX2 gene is synthetic lethal with BRCA1 and BRCA2 genes, which are required for homologous recombination (HR) repair of double-stranded DNA breaks. This is due to the ability of APEX2 to reverse single-stranded DNA breaks with 3’ end blocks. Because BRCA-deficient cancer cells are sensitive to 3’ blocks, APEX2 is a potential therapeutic target for HR-deficient cancers (2,3).

Specifications