Cell Signaling Parp (46d11) Rabbit mAb (Sepharose Bead Conjugate)

Name: Cell Signaling Parp (46d11) Rabbit mAb (Sepharose Bead Conjugate)
SKU: CSIG-6704S
Price: 454.50 USD

List Price $454.50 Your Price $454.50

Cell Signaling Parp (46d11) Rabbit mAb (Sepharose Bead Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-6704S

MFG.PART: 6704S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	400 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	116, 89
Source/Isotype	Rabbit
Application/Dilution	{Immunoprecipitation: 1:20}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Specificity/Sensitivity	PARP (46D11) Rabbit mAb (Sepharose® Bead Conjugate) detects endogenous levels of total full-length PARP and the large fragment (89 kDa) produced by caspase cleavage.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly623 of PARP protein.
Background	PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

Specifications