Cell Signaling Phospho-Stat5 (Tyr694) (D47e7) Xp® Rabbit mAb (Alexa Fluor® 555 Conjugate)

Name: Cell Signaling Phospho-Stat5 (Tyr694) (D47e7) Xp® Rabbit mAb (Alexa Fluor® 555 Conjugate)
SKU: CSIG-66740S
Price: 481.77 USD

List Price $481.77 Your Price $481.77

Cell Signaling Phospho-Stat5 (Tyr694) (D47e7) Xp® Rabbit mAb (Alexa Fluor® 555 Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-66740S

MFG.PART: 66740S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl (50 tests)
Reactivity	H M
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity/Sensitivity	Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699.
Species Reactivity	Human, Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr694 of human Stat5a protein.
Background	Stat5 is activated in response to a wide variety of ligands, including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).

Specifications