Cell Signaling Cleaved-Il-1beta (Asp117) (E7v2a) Rabbit mAb (Mouse Specific)

Name: Cell Signaling Cleaved-Il-1beta (Asp117) (E7v2a) Rabbit mAb (Mouse Specific)
SKU: CSIG-63124S
Price: 405.01 USD

List Price $405.01 Your Price $405.01

Cell Signaling Cleaved-Il-1beta (Asp117) (E7v2a) Rabbit mAb (Mouse Specific) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-63124S

MFG.PART: 63124S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	M
Sensitivity	Endogenous
Molecular Weight (kDa)	17
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Cleaved-IL-1β (Asp117) (E7V2A) Rabbit mAb recognizes endogenous levels of mouse IL-1β protein only when cleaved at Asp117.
Species Reactivity	Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp117 of mouse IL-1β protein.
Background	Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity.

Specifications