Cell Signaling Cleaved Parp (Asp214) (D64e10) Rabbit mAb (Pacific Blue™ Conjugate)

Name: Cell Signaling Cleaved Parp (Asp214) (D64e10) Rabbit mAb (Pacific Blue™ Conjugate)
SKU: CSIG-60068S
Price: 481.77 USD

List Price $481.77 Your Price $481.77

Cell Signaling Cleaved Parp (Asp214) (D64e10) Rabbit mAb (Pacific Blue™ Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-60068S

MFG.PART: 60068S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl (50 tests)
Reactivity	H Mk
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity/Sensitivity	Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Pacific Blue™ Conjugate) detects endogenous levels of the large fragment (89 kDa) of human PARP1 protein produced by caspase cleavage. The antibody does not recognize full-length PARP1 or other PARP isoforms.
Species Reactivity	Human, Monkey
Source/Purification	Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp214 of human PARP.
Background	PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

Specifications