Cell Signaling Lin28a (6d1f9) Mouse mAb

Name: Cell Signaling Lin28a (6d1f9) Mouse mAb
SKU: CSIG-5930S
Price: 339.36 USD

List Price $339.36 Your Price $339.36

Cell Signaling Lin28a (6d1f9) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-5930S

MFG.PART: 5930S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	26
Source/Isotype	Mouse IgG1
Application/Dilution	{Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:800}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	LIN28A (6D1F9) Mouse mAb detects endogenous levels of total LIN28A protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with human LIN28A recombinant protein.
Background	LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).

Specifications