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Cell Signaling Non-Phospho-Step (Ser221) (D74h3) Xp Rabbit mAb
List Price
$435.31
Your Price
$435.31
Cell Signaling Non-Phospho-Step (Ser221) (D74h3) Xp Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-5659S
MFG.PART:
5659S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H M R |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 46, 61 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Frozen): 1:50} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Non-phospho-STEP (Ser221) (D74H3) XP® Rabbit mAb detects endogenous levels of STEP61 protein only when dephosphorylated at Ser221 and of STEP46 protein when dephosphorylated at Ser49. |
| Species Reactivity | Human, Mouse, Rat |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic nonphosphopeptide corresponding to residues surrounding Ser221 of human STEP61 protein. |
| Background | Striatal enriched phosphatase (STEP, also known as PTPN5), is a protein tyrosine phosphatase expressed in dopaminoceptive neurons of the central nervous system (1). Alternative splicing produces the cytosolic STEP46 and the membrane-associated STEP61 isoforms of STEP. Dopamine activates D1 receptors and PKA, which in turn phosphorylate both isoforms of STEP. Phosphorylation of STEP61 occurs at Ser160 and Ser221, while STEP46 is phosphorylated at Ser49 (equivalent to Ser221 of STEP61) (2). NMDA-mediated activation of STEP is an important mechanism for regulation of Erk activity in neurons (3). Furthermore, STEP is involved in the regulation of both NMDAR and AMPAR trafficking (4,5). Due to its importance in cognitive function, STEP may play a role in Alzheimers disease (1).Activity of STEP61 is reduced upon phosphorylation of Ser221 (Ser49 of STEP46) due to lower affinity for its substrates (2). |
| SKU | CSIG-5659S |
|---|---|
| Featured | No |
| Supplier Part Number | 5659S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |